• Home
  • Blog
  • Article
  • Effects of DON and antidote on pro-inflammatory mRNA expression of broilers

Effects of DON and antidote on pro-inflammatory mRNA expression of broilers

 

Mycotoxins- a force to be reckoned with! 

Deoxynivalenol (DON; vomitoxin) is a trichothecene mycotoxin produced by Fusarium graminearum and is prevalently found in grains such as wheat, corn, barley, and their by-products. DON mainly affects the health of monogastric animals, particularly pigs and poultry, through contaminated feedstuffs.

Fusarium mycotoxins can be categorized into four major groups: Trichothecenes, zearalenone, fumonisins, and enniatins. 

 

Animal study

Fifty-four one-day-old broilers (Ross 308) were randomly allocated to three different treatment groups (n = 18 per group):
 

Ingredient CTRL DON DON + Antidote*
DON powder [g/kg] - 0.005 0.005
Antidote [g/kg] - - 1.5

* Antidote: Toxi-free PLUS, Life Rainbow Biotech Co., Ltd., Yilan, Taiwan.
 

Effects of DON and antidote on pro-inflammatory mRNA expression in the spleen and bursa of Fabricius of broilers

DON significantly increased COX-2 mRNA expression in the spleen and bursa compared with the control group, whereas the antidote supplement efficiently reversed the effects on COX-2 mRNA expression (Figures 1a and 2a). Although a slight increase in iNOS and IL-1β mRNA expression was observed in the DON-treated group, no statistically significant difference between the control group and the DON-treated group was found in the spleen and bursa (Figure 1b,1c, and 2b, 2c).


Figure 1. Effects of Deoxynivalenol and antidote on pro-inflammatory mRNA expression in spleen of 28-day-old broilers. (a) COX-2, (b) iNOS, (c) IL-1β and (d) TNF-α. Values were expressed as mean ± SD (n = 18). Different letters above the  bars indicate significant differences between the means (p < .05)
Figure 2. Effects of Deoxynivalenol and antidote on pro-inflammatory mRNA expression in bursa of Fabricius of 28-day-old broilers. (a) COX-2, (b) iNOS, (c) IL-1β and (d) TNF-α. Values were expressed as mean ± SD (n = 18). Different letters above the bars indicate significant differences between the means (p < .05)
 

Conclusion 

In this study, the expression of the pro-inflammatory gene, COX-2, was induced in the spleen and bursa of Fabricius of DON-fed broilers. This effect was alleviated in the presence of a mycotoxin degradation agent.
 

Reference

Yu Y. H., F. S. H. Hsiao, W. S. Proskura, A. Dybus, Y. H. Siao, Y. H. Cheng. 2018. An impact of Deoxynivalenol produced by Fusarium graminearum on broiler chickens. J. Anim. Physiol. Anim. Nutr. 2018, 102, 1012–1019.
 

Liferainbow

Liferainbow

Life Rainbow Biotech is a manufacturer with R&D of antibiotic-free feed additives. We focus on mycotoxin solution, intestinal pathogen control, immune enhancement and aqueous nutrition supplement.

Toxi-Free PLUS ®

Article Classification

Keyword Search

Subscribe to Newsletter

Name
E-mail

Article Catalog

TOP